Forecyt® software from Sartorius

Product Specs
ItemForecyt® software
CompanySartorius
Price Get Pricing Get Quote for this Product
Catalog NumberiQue Forecyt® Software
ApplicationsAdvanced Flow Cytometry
Quantity1 pack

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Local Office

Sartorius

5 Orville Drive

Bohemia, NY 11716

United States

Company Profile

International Office

Sartorius

Otto-Brenner-Straße 20

37079 Göttingen

Germany

Phone: +49 551 308 0

Company Profile

Website: www.sartorius.com

(1) “Stapling” scFv for multispecific biotherapeutics of superior propertiesmAbsDecember 31, 2023Lauren E. Boucher, Elisabeth Geyer Prinslow, Michael Feldkamp, Fang Yi, Rupesh Nanjunda, Sheng-Jiun Wu, Tun Liu, Eilyn R. Lacy, Steven Jacobs, Natalia Kozlyuk, et al.using 200 mM sodium phosphate (pH 6.8) as the running buffer. Cytotoxicity assay Intellicyt iQue3 and ForeCyt software (Sartorius) were used to determine the effect of BCMA-targeting test molecules upon T cell activation

(2) Refinement of an ovine-based immunoglobulin therapy against SARS-CoV-2, with comparison of whole IgG versus F(ab')2 fragmentsSci RepAugust 25, 2023Stephen Findlay-Wilson, Linda Easterbrook, Sandra Smith, Neville Pope, Matthew Aldridge, Gareth Humphries, Holger Schuhmann, Didier Ngabo, Emma Rayner, Ashley Otter, et al.buffer, the samples were resuspended in 40 µl HBSS and analysed using an iQue Screener Plus® with iQue Forecyt® software (Sartorius, Germany). For each sample, a minimum of 100 beads were collected. Conjugated beads were

(3) Targeting the glycine-rich domain of TDP-43 with antibodies prevents its aggregation in vitro and reduces neurofilament levels in vivoActa neuropathologica communicationsJuly 11, 2023Riemenschneider H, Simonetti F, Sheth U, Katona E, Roth S, Hutten S, Farny D, Michaelsen M, Nuscher B, Schmidt MK, et al.isotype-specific monoclonal rat anti-mouse IgG secondary antibodies. Antibody binding was analyzed using ForeCyt software (Sartorius, Germany). Clones were further validated and selected for up-scaling by sandwich ELISA. Briefly,

(4) Ex vivo venetoclax sensitivity testing predicts treatment response in acute myeloid leukemiaHaematologicaJuly 1, 2023Heikki Kuusanmäki, Sari Kytölä, Ida Vänttinen, Tanja Ruokoranta, Amanda Ranta, Jani Huuhtanen, Minna Suvela, Alun Parsons, Annasofia Holopainen, Anu Partanen, et al.Flow cytometry data were acquired using an iQue Screener PLUS instrument (Sartorius, Germany), and ForeCyt software (Sartorius) was used to analyze the cells. The gating strategy is presented in Online Supplementary

(5) Establishment and Functional Characterization of Murine Monoclonal Antibodies Recognizing NeuritinAntibodies (Basel)April 7, 2023Georgia Papadogianni, Inga Ravens, Ahmed Hassan, Andrew Flatley, Regina Feederle, Günter Bernhardt, Hristo Georgievantibodies (anti-IgG3 (clone 2E.6, HB-128, ATCC), all other in house), antibody binding was analyzed using ForeCyt software (Sartorius). Positive supernatants were further analyzed by flow cytometry analysis and selected hybridoma

(6) Syrian hamster convalescence from prototype SARS-CoV-2 confers measurable protection against the attenuated disease caused by the Omicron variantPLoS pathogensApril 1, 2023Ryan KA, Bewley KR, Watson RJ, Burton C, Carnell O, Cavell BE, Challis A, Coombes NS, Davies ER, Edun-Huges J, et al.buffer, the samples were resuspended in 40 μL HBSS and analysed using an iQue Screener Plus with iQue Forecyt software (Sartorius, Germany). For each sample, a minimum of 100 beads were collected. Conjugated beads were

(7) Pre-existing Fc profiles shape the evolution of neutralizing antibody breadth following influenza vaccinationCell Reports MedicineMarch 14, 2023Carolyn M. Boudreau, John S. Burke, Alexander L. Roederer, Matthew J. Gorman, Sophia Mundle, Daniel Lingwood, Simon Delagrave, Saranya Sridhar, Ted M. Ross, Harry Kleanthous, et al.then washed to remove unbound immune complexes, fixed, and quantified on the iQue Screener Plus using Forecyt software (Sartorius). Phagocytic scores were calculated by multiplying the percentage of bead positive cells

(8) Beneficial Effect of ACI-24 Vaccination on Aβ Plaque Pathology and Microglial Phenotypes in an Amyloidosis Mouse ModelCellsDecember 24, 2022Jasenka Rudan Njavro, Marija Vukicevic, Emma Fiorini, Lina Dinkel, Stephan A. Müller, Anna Berghofer, Chiara Bordier, Stanislav Kozlov, Annett Halle, Katrin Buschmann, et al.isotype-specific monoclonal rat-anti-mouse IgG secondary antibodies, antibody binding was analysed using ForeCyt software v9.0 (Sartorius, Goettingen, Germany). Positive supernatants were further analysed by Western blot analysis

(9) A non-canonical vitamin K cycle is a potent ferroptosis suppressorNatureAugust 25, 2022Eikan Mishima et al.Atto-488-coupled subclass-specific monoclonal mouse-anti-rat IgG. Antibody binding was analysed using ForeCyt software 8 (Sartorius). Positive supernatants were further validated by Western blotting. Selected hybridoma

(10) A novel monoclonal IgG1 antibody specific for Galactose-alpha-1,3-galactose questions alpha-Gal epitope expression by bacteriaFrontiers in ImmunologyAugust 5, 2022Luisa Kreft et al.isotype-specific monoclonal rat anti-mouse IgG secondary antibodies. Antibody binding was analyzed using ForeCyt software (Sartorius). Positive supernatants were further validated by dot blot and cells from clone 27H8 were

(11) Development of a cost-effective ovine antibody-based therapy against SARS-CoV-2 infection and contribution of antibodies specific to the spike subunit proteinsAntiviral researchJuly 1, 2022Findlay-Wilson S, Easterbrook L, Smith S, Pope N, Humphries G, Schuhmann H, Ngabo D, Rayner E, Otter AD, Coleman T, et al.buffer, the samples were resuspended in 40 μl HBSS and analysed using an iQue Screener Plus® with iQue Forecyt® software (Sartorius, Germany). For each sample, a minimum of 100 beads were collected. Conjugated beads were

(12) An optimized protocol to analyze membrane protein degradation in yeast using quantitative western blot and flow cytometrySTAR ProtocolsJune 17, 2022Felichi Mae Arines, Ming LiFor this protocol, we used the IntelliCyt® iQue Screener PLUS flow cytometer (Sartorius) and the iQue Forecyt® Software (Sartorius).Note: Other than GFP, the pH-sensitive pHluorin may also work, but for relatively abundant

(13) MS4A15 drives ferroptosis resistance through calcium-restricted lipid remodelingCell Death & DifferentiationMarch 1, 2022Shan Xin et al.isotype-specific monoclonal mouse-anti-rat IgG secondary antibodies. Antibody binding was analyzed using ForeCyt software (Sartorius). Positive supernatants were validated by western blot of Ms4a15 OE and control cell lysates.

(14) Immunotherapeutic Targeting of Mesothelin Positive Pediatric AML Using Bispecific T Cell Engaging AntibodiesCancers (Basel)November 26, 2021Anilkumar Gopalakrishnapillai et al.forward/side scatter distribution, negativity for DAPI, and positive iRFP expression using accompanying ForeCyt software. Raw cell counts were exported to Excel (Microsoft) and percent cytotoxicity was calculated utilizing

(15) Cooperativity mediated by rationally selected combinations of human monoclonal antibodies targeting the henipavirus receptor binding proteinCell ReportsAugust 31, 2021Michael P. Doyle, Nurgun Kose, Viktoriya Borisevich, Elad Binshtein, Moushimi Amaya, Marcus Nagel, Edward J. Annand, Erica Armstrong, Robin Bombardi, Jinhui Dong, et al.and algorithms GraphPad Prism 9.1 Software,"GraphPad Software, Inc.",https://www.graphpad.com iQue® Forecyt® Software,Sartorius,N/A Octet Data Acquisition 10.0.3.12 Software,ForteBio,https://www.sartorius.com/en/products/protein-analysis Octet

(16) Convergent antibody responses to the SARS-CoV-2 spike protein in convalescent and vaccinated individualsCell reportsAugust 24, 2021Chen EC, Gilchuk P, Zost SJ, Suryadevara N, Winkler ES, Cabel CR, Binshtein E, Chen RE, Sutton RE, Rodriguez J, et al.each antibody,This paper,Zenodo; DOI 10.5281/zenodo.5150784 GraphPad Prism,GraphPad,v 9.0.0 iQue® Forecyt® Software,Sartorius,N/A Nanozoomer Digital Pathology,Hamamatsu,v2 ,, Other,Other,Other ,, xCELLigence RTCA

(17) Broad and potently neutralizing monoclonal antibodies isolated from human survivors of New World hantavirus infectionCell ReportsMay 4, 2021Taylor B. Engdahl et al.Inc.",v8 FlowJo,Tree Star Inc.,v10 BioSpot 5.1 software,CTL,N/A MetaXpress,Molecular Devices,N/A iQue® Forecyt® Software,Sartorius,N/A ImMunoGeneTics (IMGT) database,"(Brochet et al., 2008); Giudicelli et al., 2011",N/A Other,Other,Other Sony

(18) Selective drug combination vulnerabilities in STAT3- and TP53-mutant malignant NK cellsBlood advancesApril 13, 2021Parri E, Kuusanmäki H, Bulanova D, Mustjoki S, Wennerberg K.room temperature before analysis using an iQue Screener PLUS flow cytometer (Sartorius, Göttingen, Germany). ForeCyt software (Sartorius) was used to gate cells and acquire population counts (as described elsewhere).27 Drug response

(19) Aminopeptidase Expression in Multiple Myeloma Associates with Disease Progression and Sensitivity to MelflufenCancers (Basel)March 26, 2021Juho J. Miettinen et al.pump speed of 32 revolutions per minute, resulting in 35 min reading time for a full 96-well plate. ForeCyt software (Sartorius) was used to gate cells and acquire population counts. Analysis was done from viable singlet

(20) Chemokine-like MDL proteins modulate flowering time and innate immunity in plantsThe Journal of biological chemistryJanuary 1, 2021Gruner K, Leissing F, Sinitski D, Thieron H, Axstmann C, Baumgarten K, Reinstädler A, Winkler P, Altmann M, Flatley A, et al.isotype-specific monoclonal mouse-anti-rat IgG secondary antibodies. Antibody binding was analyzed using ForeCyt software (Sartorius, Göttingen, Germany). Positive supernatants were further assayed for their potential by western blot

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Forecyt® software from Sartorius

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Forecyt® software from Sartorius

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